Difference between revisions of "SpoIIIAG"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
Line 90: Line 90:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[spoIIIAA]]-[[spoIIIAB]]-[[spoIIIAC]]-[[spoIIIAD]]-[[spoIIIAE]]-[[spoIIIAF]]-[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
+
* '''Operon:'''  
 +
** ''[[spoIIIAA]]-[[spoIIIAB]]-[[spoIIIAC]]-[[spoIIIAD]]-[[spoIIIAE]]-[[spoIIIAF]]-[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
 +
** ''[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]
  
* '''[[Sigma factor]]:''' [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
 
  
* '''Regulation:''' expressed during sporulation in the mother cell ([[SigE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed], switched off by [[SpoIIID]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
+
* '''[[Sigma factor]]:'''
 +
** both promoters: [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
 +
 
 +
* '''Regulation:'''  
 +
** both promoters: expressed during sporulation in the mother cell ([[SigE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed], switched off by [[SpoIIID]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
  
 
* '''Regulatory mechanism:''' [[SpoIIID]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
 
* '''Regulatory mechanism:''' [[SpoIIID]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
  
* '''Additional information:'''
+
* '''Additional information:''' the internal promoter is essential for sporulation, it is twice as active as the promoter in front of [[spoIIIAA]], suggesting that [[SpoIIIAG]] and [[SpoIIIAH]] are required in larger amounts as compared to the other products of the operon [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:42, 1 June 2009

  • Description: required for SigG activation

Gene name spoIIIAG
Synonyms
Essential no
Product unknown
Function activation of SigG
MW, pI 25 kDa, 6.407
Gene length, protein length 687 bp, 229 aa
Immediate neighbours spoIIIAH, spoIIIAF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIIIAG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: membrane protein PubMed

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation


  • Regulation:
  • Additional information: the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed