BkdAB

• Description: 2-oxoisovalerate dehydrogenase (E1 beta subunit)
  
  

Gene name	bkdAB
Synonyms	bfmBAB, bfmB1b, bkd
Essential	no
Product	2-oxoisovalerate dehydrogenase (E1 beta subunit) (2-oxoisovalerate dehydrogenase beta subunit) (EC 1.2.4.4)
Function	utilization of branched-chain keto acids
MW, pI	35 kDa, 4.791
Gene length, protein length	981 bp, 327 aa
Immediate neighbours	bkdB, bkdAA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO₂ (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: BSU24040

• E.C. number: 1.2.4.4

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

• Sigma factor: SigL PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed