Difference between revisions of "AddB"

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(Reviews)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP1106 (addAB, spc), available in [[Stülke]] lab
+
* '''Mutant:''' GP1106 ([[addA]]-[[addB]], spc), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
 
==Reviews==
 
==Reviews==
<pubmed> 23202527, 20116346 22933559 19542287 </pubmed>
+
<pubmed> 23202527, 20116346 22933559 19542287 25486468 </pubmed>
 
 
 
==Original publications==
 
==Original publications==
 
<pubmed>21809208,8387145,15610857,7746142, 19129187 1646786 10756102 9781875 17570399 20350930 22307084 22383849 21071401 23056615 24682829 24670664 8752329 </pubmed>
 
<pubmed>21809208,8387145,15610857,7746142, 19129187 1646786 10756102 9781875 17570399 20350930 22307084 22383849 21071401 23056615 24682829 24670664 8752329 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:31, 11 December 2014

  • Description: ATP-dependent deoxyribonuclease (subunit B)

Gene name addB
Synonyms
Essential no
Product ATP-dependent deoxyribonuclease (subunit B))
Function DNA repair/ recombination
Gene expression levels in SubtiExpress: addB
Interactions involving this protein in SubtInteract: AddB
MW, pI 134 kDa, 5.39
Gene length, protein length 3498 bp, 1166 aa
Immediate neighbours yhjR, addA
Sequences Protein DNA DNA_with_flanks
Genetic context
AddB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AddB expression.png















Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU10620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • the enzyme is functional as a heterodimer of the AddA and AddB subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'→5' polarity located in the AddA subunit PubMed
    • the AddB subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences PubMed
  • Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications