Difference between revisions of "BkdAB"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 272 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 2303 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:55, 17 April 2014

  • Description: 2-oxoisovalerate dehydrogenase (E1 beta subunit)

Gene name bkdAB
Synonyms bfmBAB, bfmB1b, bkd
Essential no
Product 2-oxoisovalerate dehydrogenase (E1 beta subunit)
Function utilization of branched-chain keto acids
Gene expression levels in SubtiExpress: bkdAB
Metabolic function and regulation of this protein in SubtiPathways:
bkdAB
MW, pI 35 kDa, 4.791
Gene length, protein length 981 bp, 327 aa
Immediate neighbours bkdB, bkdAA
Sequences Protein DNA DNA_with_flanks
Genetic context
BkdAB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

BkdR regulon, CodY regulon, SigL regulon

The gene

Basic information

  • Locus tag: BSU24040

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 (according to Swiss-Prot)
  • Protein family:

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of isoleucine or valine (BkdR) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 272 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2303 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References