Difference between revisions of "DnaK"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DnaK DnaK]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DnaK DnaK]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=dnaK dnaK]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 65 kDa, 4.571   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 65 kDa, 4.571   

Revision as of 11:15, 7 January 2014

  • Description: class I heat-shock protein (molecular chaperone)

Gene name dnaK
Synonyms
Essential no
Product class I heat-shock protein (molecular chaperone)
Function protein quality control
Gene expression levels in SubtiExpress: dnaK
Interactions involving this protein in SubtInteract: DnaK
Metabolic function and regulation of this protein in SubtiPathways:
dnaK
MW, pI 65 kDa, 4.571
Gene length, protein length 1833 bp, 611 aa
Immediate neighbours surC, grpE
Sequences Protein DNA DNA_with_flanks
Genetic context
DnaK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DnaK expression.png




























Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, phosphoproteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

  • Locus tag: BSU25470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: heat shock protein 70 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • membrane-proximal (Spotty) PubMed
    • recruited to the membrane after ethanol stress PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Your additional remarks

References

Additional publications: PubMed