Difference between revisions of "SpoIIR"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywlB]]'', ''[[ywlA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywlB]]'', ''[[ywlA]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36970 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36970 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36970 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36970 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36970 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36970 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:spoIIR_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:spoIIR_context.gif]]

Revision as of 11:30, 14 May 2013

  • Description: required for processing and compartmentalization of pro-SigE

Gene name spoIIR
Synonyms ipc-27d, csfX
Essential no
Product morphological chekcpoint for SigE processing
Function control of SigE activation
Gene expression levels in SubtiExpress: spoIIR
Interactions involving this protein in SubtInteract: SpoIIR
MW, pI 25 kDa, 4.737
Gene length, protein length 672 bp, 224 aa
Immediate neighbours ywlB, ywlA
Sequences Protein DNA DNA_with_flanks
Genetic context
SpoIIR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIIR expression.png
























Categories containing this gene/protein

sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

  • Locus tag: BSU36970

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: interacts with and activates the pro-SigE protease SpoIIGA in the intermembrane space between the forespore and the mother cell PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • likely acylated on Thr-27 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • localizes initially to the forespore septal membrane (in a SpoIIGA-dependent manner), is then exported to the intermembrane space between the forespore and the mother cell PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: spoIIR (according to DBTBS)
  • Regulation:
    • expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Avigdor Eldar, Vasant K Chary, Panagiotis Xenopoulos, Michelle E Fontes, Oliver C Losón, Jonathan Dworkin, Patrick J Piggot, Michael B Elowitz
Partial penetrance facilitates developmental evolution in bacteria.
Nature: 2009, 460(7254);510-4
[PubMed:19578359] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Kit Pogliano
Septal localization of forespore membrane proteins during engulfment in Bacillus subtilis.
EMBO J: 2004, 23(7);1636-46
[PubMed:15044948] [WorldCat.org] [DOI] (P p)

Masaya Fujita, Richard Losick
An investigation into the compartmentalization of the sporulation transcription factor sigmaE in Bacillus subtilis.
Mol Microbiol: 2002, 43(1);27-38
[PubMed:11849534] [WorldCat.org] [DOI] (P p)

A E Hofmeister, A Londoño-Vallejo, E Harry, P Stragier, R Losick
Extracellular signal protein triggering the proteolytic activation of a developmental transcription factor in B. subtilis.
Cell: 1995, 83(2);219-26
[PubMed:7585939] [WorldCat.org] [DOI] (P p)