Difference between revisions of "DnaK"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[surC]]'', ''[[grpE]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[surC]]'', ''[[grpE]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25470 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25470 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25470 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25470 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25470 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25470 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:dnaK_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:dnaK_context.gif]]

Revision as of 10:45, 14 May 2013

  • Description: class I heat-shock protein (molecular chaperone)

Gene name dnaK
Synonyms
Essential no
Product class I heat-shock protein (molecular chaperone)
Function protein quality control
Gene expression levels in SubtiExpress: dnaK
Interactions involving this protein in SubtInteract: DnaK
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 65 kDa, 4.571
Gene length, protein length 1833 bp, 611 aa
Immediate neighbours surC, grpE
Sequences Protein DNA DNA_with_flanks
Genetic context
DnaK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DnaK expression.png




























Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, phosphoproteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

  • Locus tag: BSU25470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: heat shock protein 70 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • membrane-proximal (Spotty) PubMed
    • recruited to the membrane after ethanol stress PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Your additional remarks

References

Additional publications: PubMed

Christine Eymann, Dörte Becher, Jörg Bernhardt, Katrin Gronau, Anja Klutzny, Michael Hecker
Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.
Proteomics: 2007, 7(19);3509-26
[PubMed:17726680] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Dindo Y Reyes, Hirofumi Yoshikawa
DnaK chaperone machine and trigger factor are only partially required for normal growth of Bacillus subtilis.
Biosci Biotechnol Biochem: 2002, 66(7);1583-6
[PubMed:12224648] [WorldCat.org] [DOI] (P p)

G Homuth, A Mogk, W Schumann
Post-transcriptional regulation of the Bacillus subtilis dnaK operon.
Mol Microbiol: 1999, 32(6);1183-97
[PubMed:10383760] [WorldCat.org] [DOI] (P p)

G Homuth, S Masuda, A Mogk, Y Kobayashi, W Schumann
The dnaK operon of Bacillus subtilis is heptacistronic.
J Bacteriol: 1997, 179(4);1153-64
[PubMed:9023197] [WorldCat.org] [DOI] (P p)

G Yuan, S L Wong
Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK.
J Bacteriol: 1995, 177(22);6462-8
[PubMed:7592421] [WorldCat.org] [DOI] (P p)

A Schulz, B Tzschaschel, W Schumann
Isolation and analysis of mutants of the dnaK operon of Bacillus subtilis.
Mol Microbiol: 1995, 15(3);421-9
[PubMed:7540247] [WorldCat.org] [DOI] (P p)

M Wetzstein, U Völker, J Dedio, S Löbau, U Zuber, M Schiesswohl, C Herget, M Hecker, W Schumann
Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.
J Bacteriol: 1992, 174(10);3300-10
[PubMed:1339421] [WorldCat.org] [DOI] (P p)