YqeV

• Description: tRNA methylthiotransferase
  
  

Gene name	yqeV
Synonyms
Essential	no
Product	tRNA methylthiotransferase
Function	tRNA maturation
Gene expression levels in SubtiExpress: yqeV
MW, pI	51 kDa, 5.242
Gene length, protein length	1353 bp, 451 aa
Immediate neighbours	yqeW, yqeU
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, heat shock proteins, membrane proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

• Locus tag: BSU25430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA PubMed

• Protein family: YqeV subfamily (according to Swiss-Prot)

• Paralogous protein(s): YmcB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): 2 (4Fe-4S) clusters PubMed

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54462

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Expression browser: yqeV PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat shock (HrcA) PubMed

• Regulatory mechanism:
  • HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Simon Arragain, Samuel K Handelman, Farhad Forouhar, Fan-Yan Wei, Kazuhito Tomizawa, John F Hunt, Thierry Douki, Marc Fontecave, Etienne Mulliez, Mohamed Atta
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
J Biol Chem: 2010, 285(37);28425-33
[PubMed:20584901] [WorldCat.org] [DOI] (I p)

Brian P Anton, Susan P Russell, Jason Vertrees, Simon Kasif, Elisabeth A Raleigh, Patrick A Limbach, Richard J Roberts
Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis.
Nucleic Acids Res: 2010, 38(18);6195-205
[PubMed:20472640] [WorldCat.org] [DOI] (I p)

G Homuth, A Mogk, W Schumann
Post-transcriptional regulation of the Bacillus subtilis dnaK operon.
Mol Microbiol: 1999, 32(6);1183-97
[PubMed:10383760] [WorldCat.org] [DOI] (P p)

G Homuth, S Masuda, A Mogk, Y Kobayashi, W Schumann
The dnaK operon of Bacillus subtilis is heptacistronic.
J Bacteriol: 1997, 179(4);1153-64
[PubMed:9023197] [WorldCat.org] [DOI] (P p)

G Yuan, S L Wong
Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK.
J Bacteriol: 1995, 177(22);6462-8
[PubMed:7592421] [WorldCat.org] [DOI] (P p)