Difference between revisions of "AddB"

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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1Y26 1Y26] (the [[riboswitch]] in complex with adenine)  
+
* '''Structure:'''  
 +
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=3U4Q 3U4Q] (the [[AddA]]-[[AddB]]-DNA complex) {{PubMed|22307084}}
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** [http://www.rcsb.org/pdb/explore.do?structureId=1Y26 1Y26] (the [[riboswitch]] in complex with adenine)  
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P23477 P23477]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P23477 P23477]
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==Original publications==
 
==Original publications==
 
'''Additional publications:''' {{PubMed|21071401}}
 
'''Additional publications:''' {{PubMed|21071401}}
<pubmed>21809208,8387145,15610857,7746142, 19129187 1646786 10756102 9781875 17570399 20350930 </pubmed>
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<pubmed>21809208,8387145,15610857,7746142, 19129187 1646786 10756102 9781875 17570399 20350930 22307084</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:26, 4 May 2012

  • Description: ATP-dependent deoxyribonuclease (subunit B)

Gene name addB
Synonyms
Essential no
Product ATP-dependent deoxyribonuclease (subunit B))
Function DNA repair/ recombination
Interactions involving this protein in SubtInteract: AddB
MW, pI 134 kDa, 5.39
Gene length, protein length 3498 bp, 1166 aa
Immediate neighbours yhjR, addA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AddB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AddB expression.png


























Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU10620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • the enzyme is functional as a heterodimer of the AddA and AddB subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'→5' polarity located in the AddA subunit PubMed
    • the AddB subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences PubMed
  • Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1106 (addAB, spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Joseph T P Yeeles, Mark S Dillingham
The processing of double-stranded DNA breaks for recombinational repair by helicase-nuclease complexes.
DNA Repair (Amst): 2010, 9(3);276-85
[PubMed:20116346] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed