Difference between revisions of "BioB"

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Revision as of 09:16, 16 July 2011

  • Description: biotin synthase

Gene name bioB
Synonyms
Essential no
Product biotin synthase)
Function biosynthesis of biotin
Metabolic function and regulation of this protein in SubtiPathways:
Biotin
MW, pI 36 kDa, 5.554
Gene length, protein length 1005 bp, 335 aa
Immediate neighbours bioI, bioD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BioB context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

  • Locus tag: BSU30200

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot)
  • Protein family: Biotin synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): contains an iron-sulfur cluster
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

  • Regulation:
    • repressed in the presence of biotin (BirA) PubMed
  • Regulatory mechanism:
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original publications

Ulf Gerth, Holger Kock, Ilja Kusters, Stephan Michalik, Robert L Switzer, Michael Hecker
Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis.
J Bacteriol: 2008, 190(1);321-31
[PubMed:17981983] [WorldCat.org] [DOI] (I p)

J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842] [WorldCat.org] [DOI] (P p)

S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940] [WorldCat.org] [DOI] (P p)