ResD

• Description: two-component response regulator, regulation of aerobic and anaerobic respiration
  
  

Gene name	resD
Synonyms	ypxD
Essential	no
Product	two-component response regulator
Function	regulation of aerobic and anaerobic respiration respiration
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Central C-metabolism, Stress
MW, pI	27 kDa, 5.631
Gene length, protein length	720 bp, 240 aa
Immediate neighbours	resE, resC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23120

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

regulators of electron transport, transcription factors and their control, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: OmpR family of two-component response regulators

• Paralogous protein(s):

Genes/ operons regulated by ResD

nrdI-nrdE-nrdF-ymaB PubMed, resA, ctaA, qcrA-qcrB-qcrC, fnr, lctE-lctP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by ResE on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions: ResE-ResD

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P35163

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • resA-resB-resC-resD-resE PubMed
  • resD-resE PubMed

• Sigma factor:
  • resA: SigA PubMed
  • resD: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed
  • PhoP: transcription activation PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage