Difference between revisions of "LevE"
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| − | * '''Description:''' [[trigger enzymes|trigger enzyme]]: fructose-specific phosphotransferase system, EIIB component <br/><br/> | + | * '''Description:''' [[trigger enzymes|trigger enzyme]]: fructose-specific [[PTS|phosphotransferase system]], EIIB component of the [[PTS]]<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: fructose-specific phosphotransferase system, EIIB component | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: fructose-specific [[PTS|phosphotransferase system]], EIIB component |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation, control of LevR activity | |style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation, control of LevR activity | ||
| Line 56: | Line 56: | ||
* '''Catalyzed reaction/ biological activity:''' Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot) | * '''Catalyzed reaction/ biological activity:''' Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot) | ||
| − | * '''Protein family:''' PTS permease, mannose permease (Man) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed] | + | * '''Protein family:''' [[PTS]] permease, mannose permease (Man) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed] |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
Revision as of 19:22, 24 November 2009
- Description: trigger enzyme: fructose-specific phosphotransferase system, EIIB component of the PTS
| Gene name | levE |
| Synonyms | |
| Essential | no |
| Product | trigger enzyme: fructose-specific phosphotransferase system, EIIB component |
| Function | fructose uptake and phosphorylation, control of LevR activity |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 17 kDa, 9.444 |
| Gene length, protein length | 486 bp, 162 aa |
| Immediate neighbours | levF, levD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU27060
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure: 1BLE
- UniProt: P26380
- KEGG entry: [2]
- E.C. number: 2.7.1.69
Additional information
Expression and regulation
- Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose
- Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
