polY1
168
translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci
locus
BSU_23870
Molecular weight
46.86 kDa
pI
9.95
function
generation of mutations in stationary phase
product
translesion synthesis (TLS-) DNA polymerase Y1
essential
no
ec
2.7.7.7
synonyms
polY1, yqjH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0389 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,482,269 → 2,483,513
Phenotypes of a mutant
reduced UV-induced mutagenesis [Pubmed|12644484]
sensitive to blue light-induced DNA damage [pubmed|30054368]
increased sensitivity to Cr(VI) [pubmed|30745368]
The protein
Catalyzed reaction/ biological activity
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
required for Cr(VI)-induced mutagenesis [pubmed|30745368]
Protein family
[wiki|DNA polymerase type-Y family] (according to UniProt)
[wiki|Domains]
[wiki|UmuC domain] (aa 8-189) (according to UniProt)
Structure
[PDB|4IRC] (from E. coli, 35% identity) [pubmed|23525461]
[AF|P54545]
Paralogous protein(s)
[protein|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2], [protein|BE8D5E038C0FD748C0F9C3F18DCA601BFFE8EA52|uvrX]
[wiki|Localization]
cytoplasm, moderately enriched near sites of replication during normal growth [pubmed|39051562]
Expression and Regulation
Operons
genes
[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]-[gene|61B7C51EB7E74226890010B61D8E41C02189A453|gndA]
description
[pubmed|22383849]
regulation
constitutively expressed [Pubmed|15469515]
Biological materials
Mutant
MGNA-C390 (yqjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2388 NBRP B. subtilis, Japan]
GP1111 (spc), available in [wiki|Jörg Stülke]'s lab [pubmed|31948638]
BKE23870 (Δ[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG
BKK23870 (Δ[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG
References
Reviews
Original Publications
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Time of last update: 2025-04-01 22:11:52
Author of last update: Jstuelk