nhaK

nhaK
168

cation/H+ antiporter

Locus
BSU_33420
Molecular weight
75.05 kDa
Isoelectric point
9.22
Protein length
670 aaSequenceBlast
Gene length
2013 bpSequenceBlast
Function
monovalent cation export
Product
cation/H+ antiporter
Essential
no
Synonyms
nhaK, yvgP
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0025 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,428,331  3,430,343
Phenotypes of a mutant
specific gain-of-function point mutations affecting NhaK facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing potassium or glutamate, these mutations enhance the activity of NhaK in potassium export PubMed
The protein
Catalyzed reaction/ biological activity
export of Na+, K+, Li+, Rb+
Protein family
monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family (single member, according to UniProt)
Structure
O32212 (AlphaFold)
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
nhaK
Description
PubMed
Regulation
constitutive, expression increases in the presence of Na+ and K+ PubMed
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nhaK

2025-03-06 07:48:42

Jstuelk

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fd9f529f4ce3208805eb406fb99847b87146a5f3

9C62C26CD4859D9C5CD4CB478377450237DA851B

Biological materials
Mutant
MGNA-A455 (yvgP::erm), available at the NBRP B. subtilis, Japan
GP2226 (''nhaK::aphA3''), available in Jörg Stülke's lab PubMed
BKE33420 (nhaK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATGCATGACCTCCTTCG,  downstream forward: _UP4_TAAATGCAGAAAAAAAGCTG
BKK33420 (nhaK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATGCATGACCTCCTTCG,  downstream forward: _UP4_TAAATGCAGAAAAAAAGCTG
FLAG-tag construct
GP2436 nhaK-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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17F0D94ED61E0AF1015FBC845BC2B5B670311C6F

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Time of last update: 2025-03-17 20:09:00

Author of last update: Jstuelk