nhaK

nhaK
168

cation/H+ antiporter

Locus
BSU_33420
Molecular weight
75.05 kDa
Isoelectric point
9.22
Protein length
Gene length
Function
monovalent cation export
Product
cation/H+ antiporter
Essential
no
Synonyms
nhaK, yvgP

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0025 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,428,331  3,430,343
Phenotypes of a mutant
specific gain-of-function point mutations affecting NhaK facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing potassium or glutamate, these mutations enhance the activity of NhaK in potassium export PubMed
The protein
Catalyzed reaction/ biological activity
export of Na+, K+, Li+, Rb+
Protein family
monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family (single member, according to UniProt)
Structure
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
Description
Regulation
constitutive, expression increases in the presence of Na+ and K+ PubMed
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nhaK

2025-03-06 07:48:42

Jstuelk

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fd9f529f4ce3208805eb406fb99847b87146a5f3

9C62C26CD4859D9C5CD4CB478377450237DA851B

Biological materials
Mutant
MGNA-A455 (yvgP::erm), available at the NBRP B. subtilis, Japan
GP2226 (''nhaK::aphA3''), available in Jörg Stülke's lab PubMed
BKE33420 (nhaK::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAATGCATGACCTCCTTCG,  downstream forward: _UP4_TAAATGCAGAAAAAAAGCTG
BKK33420 (nhaK::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAATGCATGACCTCCTTCG,  downstream forward: _UP4_TAAATGCAGAAAAAAAGCTG
FLAG-tag construct
GP2436 nhaK-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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17F0D94ED61E0AF1015FBC845BC2B5B670311C6F

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Time of last update: 2025-03-17 20:09:00

Author of last update: Jstuelk