nhaK
168
cation/H+ antiporter
locus
BSU_33420
Molecular weight
75.05 kDa
pI
9.22
function
monovalent cation export
product
cation/H+ antiporter
essential
no
synonyms
nhaK, yvgP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0025 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,428,331 3,430,343
Phenotypes of a mutant
specific gain-of-function point mutations affecting [protein|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK] facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing potassium or glutamate, these mutations enhance the activity of [protein|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK] in potassium export [pubmed|28420751,33481774]
The protein
Catalyzed reaction/ biological activity
export of Na+, K+, Li+, Rb+
Protein family
monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family (single member, according to UniProt)
Structure
[AF|O32212]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK]
description
[Pubmed|16021482]
regulation
constitutive, expression increases in the presence of Na+ and K+ [Pubmed|16021482]
Biological materials
Mutant
MGNA-A455 (yvgP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/455 NBRP B. subtilis, Japan]
GP2226 (''[gene|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK]''::''aphA3''), available in [wiki|Jörg Stülke]'s lab [pubmed|28420751]
BKE33420 ([gene|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGCATGACCTCCTTCG, downstream forward: _UP4_TAAATGCAGAAAAAAAGCTG
BKK33420 ([gene|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGCATGACCTCCTTCG, downstream forward: _UP4_TAAATGCAGAAAAAAAGCTG
FLAG-tag construct
GP2436 ''nhaK-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-21 11:14:19
Author of last update: Jstuelk