rpmEB
168
general stress protein, binds in the stationary phase to the [wiki|ribosome], replaces [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE] under conditions of zinc limitation
locus
BSU_30700
Molecular weight
9.39 kDa
pI
9.81
function
survival of salt stress
product
accessory ribosomal protein
essential
no
synonyms
rpmEB, rpmE2, ytiA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0254 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,138,978 → 3,139,226
The protein
Protein family
bacterial [wiki|ribosomal protein] bL31 family (with [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE], according to UniProt)
Structure
[PDB|5O5J] (30S ribosome subunit of Mycobacterium smegmatis, 43% identity) [pubmed|28683309]
[AF|O34967]
Modification
phosphorylated on Arg-70 [Pubmed|22517742]
Paralogous protein(s)
[protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
Expression and Regulation
Operons
genes
[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]
description
[Pubmed|22383849]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528]
regulatory mechanism
[protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]: repression, in the presence of zinc [PubMed|12904577,15049826], in [regulon|protein:A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-A292 (ytiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/292 NBRP B. subtilis, Japan]
BKE30700 (Δ[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCTCCTTTCAAT, downstream forward: _UP4_TAAGGCAGGCCTGAGGGTTT
BKK30700 (Δ[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCTCCTTTCAAT, downstream forward: _UP4_TAAGGCAGGCCTGAGGGTTT
References
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Time of last update: 2024-12-21 20:38:16
Author of last update: Jstuelk