rpmEB

rpmEB
168

general stress protein, binds in the stationary phase to the [wiki|ribosome], replaces [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE] under conditions of zinc limitation

locus
BSU_30700
Molecular weight
9.39 kDa
pI
9.81
Protein length
Gene length
function
survival of salt stress
product
accessory ribosomal protein
essential
no
synonyms
rpmEB, rpmE2, ytiA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0254 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,138,978 → 3,139,226
The protein
Protein family
bacterial [wiki|ribosomal protein] bL31 family (with [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE], according to UniProt)
Structure
[PDB|5O5J] (30S ribosome subunit of Mycobacterium smegmatis, 43% identity) [pubmed|28683309]
[AF|O34967]
Modification
phosphorylated on Arg-70 [Pubmed|22517742]
Paralogous protein(s)
[protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
Expression and Regulation
Operons
genes
[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]
description
[Pubmed|22383849]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528]
regulatory mechanism
[protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]: repression, in the presence of zinc [PubMed|12904577,15049826], in [regulon|protein:A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
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[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]

2024-11-16 07:16:08

ghost

60

b4c7f062bb2bd44876d1f81bcd37e6590446e62b

DDD400592362E594DF226AA918BD00E3DE08FCF7

Biological materials
Mutant
MGNA-A292 (ytiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/292 NBRP B. subtilis, Japan]
BKE30700 (Δ[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCTCCTTTCAAT,  downstream forward: _UP4_TAAGGCAGGCCTGAGGGTTT
BKK30700 (Δ[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCTCCTTTCAAT,  downstream forward: _UP4_TAAGGCAGGCCTGAGGGTTT
References
36815589,17163968,15049826,16547061,12904577,22383849,15805528,19648245,22517742,23002217,27561249,28683309

DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A

Page visits: 4930

Time of last update: 2024-12-05 08:06:21

Author of last update: Jstuelk