ykkE
168
[metabolite|formyltetrahydrofolate] deformylase
locus
BSU_13110
Molecular weight
34.34 kDa
pI
5.95
function
purine nucleotide synthesis
product
[metabolite|formyltetrahydrofolate] deformylase
essential
no
ec
3.5.1.10
synonyms
ykkE, purU
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0788 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,377,243 1,378,145
The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + H2O --> (6S)-5,6,7,8-tetrahydrofolate + [metabolite|formate] + H+ (according to UniProt)
Protein family
PurU family (single member, according to UniProt)
[wiki|Domains]
[wiki|ACT domain] (aa 21-102) (according to UniProt)
Structure
[PDB|3W7B] (from ''Thermus thermophilus'', 58% identity) [Pubmed|24108189]
[AF|O34990]
Paralogous protein(s)
[protein|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]
Expression and Regulation
Operons
genes
[gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B310 (ykkE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1309 NBRP B. subtilis, Japan]
BKE13110 ([gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG
BKK13110 ([gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG
References
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Time of last update: 2025-01-13 20:42:23
Author of last update: Jstuelk