pabA
168
para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II), glutaminase subunit
locus
BSU_00750
Molecular weight
21.54 kDa
pI
4.78
function
biosynthesis of folate and tryptophan
product
para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II) glutamine amidotransferase (subunit B) and anthranilate synthase (subunit II)
essential
no
ec
2.6.1.85
synonyms
pabA, trpG, trpX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0512 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
84,290 84,874
The protein
Catalyzed reaction/ biological activity
[metabolite|chorismate] + L-[protein|search|glutamine] --> [protein|search|anthranilate] + [protein|search|pyruvate] + L-[protein|search|glutamate] (according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-1 domain] (aa 1-194) (according to UniProt)
Structure
[PDB|6QUR] (synthetic construct, 72.9% identity) [pubmed|31121180]
[PDB|1I1Q] (from ''Salmonella typhimurium'', 42% identity) [Pubmed|11224570]
[AF|P28819]
Expression and Regulation
Operons
genes
[gene|B189AD3E3D18876E1956763182D835A151727392|pabB]-[gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC]-[gene|FA9539CB701BC47FD4E849FBA6D7B201B27C6104|sul]-[gene|AAE44B4F73C7C02F7DB3574B6169E0DE46F4B787|folB]-[gene|D2B189C6292ACE1B25658B407C284E9A683EAE5B|folK]-[gene|F373EBC35805DC551E8AFF6FDC7E43D5587C4700|yazB]-[gene|CE45D56BC413555AF4E7914B5307AD967A358452|dusB1]-[gene|24CC89DE55B3C19F80B11D106B9FD241174AE3E4|lysS]
description
[Pubmed|9084182]
regulation
translation repressed by tryptophan ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|9084182]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9084182], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Other regulations
[protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: translation inhibition, [Pubmed|9084182]
additional information
the [gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC] part of the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
BKE00750 ([gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATCATTTCTCCGC, downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
BKK00750 ([gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATCATTTCTCCGC, downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
References
Reviews
Original Publications
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Time of last update: 2025-01-29 07:42:59
Author of last update: Jstuelk