ywlF
168
ribose-5-phosphate isomerase
locus
BSU_36920
Molecular weight
16.07 kDa
pI
5.42
function
pentose phosphate pathway
product
ribose-5-phosphate isomerase
essential
no
ec
5.3.1.-
synonyms
ywlF, ipc-32d
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0698 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,791,199 3,791,648
Phenotypes of a mutant
poor growth [pubmed|28189581]
poorly transformable [pubmed|28189581]
The protein
Protein family
LacAB/RpiB family (single member, according to UniProt)
Structure
[PDB|3HEE] (from ''Clostridium thermocellum'', 55% identity, 72% similarity) [pubmed|21253719]
[AF|P39156]
Effectors of protein activity
Inhibited by 6-phosphogluconate and AMP [Pubmed|7144591]
Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ [Pubmed|956158]
Activated by EDTA and 2-mercaptoethanol [Pubmed|7144591]
Kinetic information
Reversible Mass Action Kinetics [Pubmed|956158] [Pubmed|7144591]
Additional information
The enzyme has probably more than one active site, but with no cooperativity described [Pubmed|7144591]
Expression and Regulation
Operons
genes
[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
description
[Pubmed|12823818]
regulation
repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|12823818], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
genes
[gene|19829F6D993188BE854F0F61CB93777AD04CF754|ywlD]-[gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
description
[Pubmed|22383849]
genes
[gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE36920 ([gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGCTTTTCCTCCCAGC, downstream forward: _UP4_AAAAACCTGTAGGGGTGCTG
BKK36920 ([gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGCTTTTCCTCCCAGC, downstream forward: _UP4_AAAAACCTGTAGGGGTGCTG
FLAG-tag construct
GP1404 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
Antibody
**
References
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Time of last update: 2024-12-22 00:49:12
Author of last update: Melvin.boenninger