prkA
168
AAA+ ATP-dependent protease
locus
BSU_08970
Molecular weight
72.71 kDa
pI
5.46
function
control of [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]-dependent gene expression
product
AAA+ ATP-dependent protease
essential
no
synonyms
prkA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2766 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
973,156 975,051
Phenotypes of a mutant
sporulation frequency reduced to 12-27% in knockout [Pubmed|36041628,12662922], due to reduced [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK] expression, results from strong repression by [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC] [Pubmed|25983726]
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes ATP [pubmed|36041628]
inactivation of [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC] [Pubmed|25983726]
Protein family
prkA family (single member, according to UniProt)
Structure
[AF|P39134]
Modification
phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] on The-217 [pubmed|36041628]
[wiki|Localization]
spore coat [Pubmed|25983726]
Additional information
[http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=G6969-MONOMER the corresponding protein from ''E. coli'' (YeaG)]
Expression and Regulation
Operons
genes
[gene|83F1BE86A7AE1098C1319198A8DDA6B8C5F49236|prkA]
description
[Pubmed|8626065]
regulation
repressed by casamino acids [Pubmed|12107147]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE08970 ([gene|83F1BE86A7AE1098C1319198A8DDA6B8C5F49236|prkA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGGACCTCCTCTATCA, downstream forward: _UP4_TAAGCAACGTGACCCGTCCG
BKK08970 ([gene|83F1BE86A7AE1098C1319198A8DDA6B8C5F49236|prkA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGGACCTCCTCTATCA, downstream forward: _UP4_TAAGCAACGTGACCCGTCCG
References
Reviews
Original Publications
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Time of last update: 2024-12-21 06:21:47
Author of last update: Dennis.wicke