cotA
168
laccase, bilirubin oxidase, spore coat protein (outer)
locus
BSU_06300
Molecular weight
58.33 kDa
pI
5.89
function
resistance of the spore
product
laccase, bilirubin oxidase
essential
no
ec
1.3.3.5
synonyms
cotA, pig
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2132 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
683,462 685,003
The protein
Protein family
S.antibioticus phenoxazinone synthase (PhsA)
[wiki|Cofactors]
Cu(I)
Structure
[PDB|2BHF] (reduced form)
[AF|P07788]
[wiki|Localization]
outer spore coat, more abundant at the mother cell-distal pole of the forespore, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] [Pubmed|22171814,19933362]
Expression and Regulation
Operons
genes
[gene|73473E6556D374C8623A426E075038AD01AB7025|cotA]
description
[Pubmed|22383849]
regulation
expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: repression, [Pubmed|15383836], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,3135411], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
additional information
[protein|search|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
1S101 ( ''cotA''::''cat''), [Pubmed|2821284], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S101&Search=1S101 BGSC]
1S134 ( ''cotA''::''kan''), [Pubmed|11514528], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S134&Search=1S134 BGSC]
BKE06300 ([gene|73473E6556D374C8623A426E075038AD01AB7025|cotA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCTGTCCTTATC, downstream forward: _UP4_TAACCCGACAAACTTGCCTC
BKK06300 ([gene|73473E6556D374C8623A426E075038AD01AB7025|cotA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCTGTCCTTATC, downstream forward: _UP4_TAACCCGACAAACTTGCCTC
References
Reviews
Original Publications
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Time of last update: 2024-12-20 16:16:39
Author of last update: Jstuelk