ribU
168
riboflavin [wiki|ECF transporter], S protein
locus
BSU_23050
Molecular weight
20.41 kDa
pI
10.12
function
riboflavin uptake
product
S protein of riboflavin [wiki|ECF transporter]
essential
no
synonyms
ribU, ypaA, fmnP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3601 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,410,017 2,410,589
The protein
Protein family
prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family (single member, according to UniProt)
Structure
[PDB|3P5N] (RibU from ''Staphylococcus aureus'')
[AF|P50726]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]
description
[Pubmed|21815947]
regulation
expressed in the absence of FMN ([protein|search|FMN-box]) [Pubmed|17693491]
antitermination is modulated by [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]-dependent transcription pausing just upstream of the [protein|search|FMN-box] [pubmed|39494516]
binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR] to the [wiki|FMN-box] riboswitch can enforce expression even in the presence of FMN [pubmed|26494285]
regulatory mechanism
FMN-box: RNA switch, via [protein|C245476CE35028167510F67153F392D91447553F|mgtE riboswitch], sequestration of the Shine-Dalgarno sequence in the presence of FMN or FMNH2, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR], in [regulon|other_regulator:FMN-box|FMN-box]
[protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]: antitermination, [pubmed|26494285], in [regulon|protein:3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR regulon]
additional information
the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
GP4785 (trpC2 Δ[gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::neo), available in [wiki|Jörg Stülke]'s lab
MGNA-A478 (ypaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/478 NBRP B. subtilis, Japan]
BKE23050 ([gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC, downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT
BKK23050 ([gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC, downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT
References
Reviews
Original Publications
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Time of last update: 2024-11-21 04:01:38
Author of last update: Jstuelk