yrkA

yrkA
168

similar to magnesium exporter

Locus
BSU_26610
Molecular weight
48.82 kDa
Isoelectric point
4.92
Protein length
434 aaSequenceBlast
Gene length
1305 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
yrkA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,718,959  2,720,263
The protein
Protein family
UPF0053 family(according to UniProt)
Domains
CNNM transmembrane domain (aa 1-201) (according to UniProt)
DUF21 domain (5-201)
2 CBS domains (aa 220-282, aa 289-346) (according to UniProt)
Structure
4HG0 (PDB) (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain)
P54428 (AlphaFold)
Paralogous protein(s)
YugS, YhdT, MpfA, YqhB
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
yrkA
Description
PubMed
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yrkA

2025-04-05 03:10:15

Jstuelk

53

bd7a678b481d8d51ddc92dcb6c0c7be0c7cc577e

4116F56C6ED0B1844C6B1D4276A0D5762B58DBCE

Biological materials
Mutant
BKE26610 (yrkA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGATTTGCCTTACCTAG,  downstream forward: _UP4_TAACATCTGGATAAAACACA
BKK26610 (yrkA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGATTTGCCTTACCTAG,  downstream forward: _UP4_TAACATCTGGATAAAACACA
Expression vectors
pGP2932 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3465 (214-350aa) (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3470 (214-350aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
References
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Page visits: 2494

Time of last update: 2025-04-08 02:09:01

Author of last update: LKrueger