yrkA
168
similar to magnesium exporter
locus
BSU_26610
Molecular weight
48.82 kDa
pI
4.92
function
unknown
product
unknown
essential
no
synonyms
yrkA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,718,959 2,720,263
The protein
Protein family
[wiki|UPF0053 family](according to UniProt)
[wiki|Domains]
[wiki|CNNM transmembrane domain] (aa 1-201) (according to UniProt)
[wiki|DUF21 domain] (5-201)
2 [wiki|CBS domain]s (aa 220-282, aa 289-346) (according to UniProt)
Structure
[PDB|4HG0] (CorC from E. coli, corresponds to the [wiki|CBS domain]s and the C-terminal transporter-associated domain)
[AF|P54428]
Paralogous protein(s)
[protein|A459410312A926365B45CEB4694DE399B38820A2|yugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|yhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA], [protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE26610 ([gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGATTTGCCTTACCTAG, downstream forward: _UP4_TAACATCTGGATAAAACACA
BKK26610 ([gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGATTTGCCTTACCTAG, downstream forward: _UP4_TAACATCTGGATAAAACACA
Expression vectors
pGP2932 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3465 (214-350aa) (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3470 (214-350aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
References
Page visits: 2154
Time of last update: 2024-10-14 18:30:58
Author of last update: LKrueger