hfq

hfq
168

RNA chaperone

locus
BSU_17340
Molecular weight
8.38 kDa
pI
8.7
Protein length
73 aaSequenceBlast
Gene length
222 bpSequenceBlast
function
unknown
product
RNA chaperone
essential
no
synonyms
hfq, ymaH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1923 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,867,373  1,867,594
The protein
Protein family
hfq family (single member, according to UniProt)
Structure
[PDB|3HSB] (complex with an RNA aptamer) [Pubmed|22053080]
[AF|O31796]
Expression and Regulation
Operons
genes
[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[pubmed|22383849]
Open in new tab

[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]→[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

2025-03-16 08:15:34

Jstuelk

113

618ff8dc028666b7102a8ac70fd2e0e7e7cee80a

30921149A989DB9C49A175051521F780FA3F893A

genes
[gene|61B511C49912F1380AEE18AD300255A435F56F94|gerY]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[pubmed|22383849]
regulation
expressed during [wiki|sporulation ] in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|39530705,22383849]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [pubmed|39530705], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Open in new tab

[gene|61B511C49912F1380AEE18AD300255A435F56F94|gerY]→[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

2025-03-16 01:24:45

Jstuelk

122

4C22FBFE04A5390FCBAE1A84C6631B5B7D9CC3D4

414C28CC98280BCBBDFD5DED7555018FF98C974C

genes
[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[Pubmed|23457461]
regulation
expression (mRNA levels) is quite constant during growth in minimal medium [Pubmed|23457461]
Open in new tab

[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

2025-03-07 10:14:06

ghost

90

5dd5c30b8f03a039a028cd06c3c722fb9d061cf7

9591F91B91F9458EDFFD1833176867742AF16A21

Biological materials
Mutant
GP22 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::cat), available in [wiki|Jörg Stülke]'s lab
BKE17340 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17340 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT,  downstream forward: _UP4_TAGATCAAAAAATGCCATGT
BKK17340 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17340 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT,  downstream forward: _UP4_TAGATCAAAAAATGCCATGT
Expression vectors
pGP2166: expression of ''hfq''-Strep by [wiki|pGP382] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1067 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP460 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
17395525,15009882,21760622,26773797,32850966,34372700,33690958,38052429
Original Publications
12850135,20445260,23457461,22965117,22053080,24932523,25150227,25915524,27049793,27581927,12093755,34129180,35614284

40D3FE1A512703B090316C31099956DB6A7F515B

Page visits: 2266

Time of last update: 2025-04-01 18:30:41

Author of last update: Jstuelk