tlpA
168
membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, acts also as pH sensor, responds to decreasing pH as an attractant signal
locus
BSU_31250
Molecular weight
72.09 kDa
pI
4.91
function
control of chemotaxis, sensing of acidic environments
product
methyl-accepting chemotaxis protein
essential
no
synonyms
tlpA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,208,280 3,210,268
The protein
[wiki|Domains]
[wiki|Cache domain] (aa 152-228) (according to UniProt)
[wiki|HAMP domain] (aa 303-355) (according to UniProt)
[wiki|Methyl-accepting transducer domain] (aa 374-610) (according to UniProt)
Structure
[PDB|6S1K] (from E. coli, corresponds to aa 285 ... 552, 33% identity) [pubmed|31925330]
[AF|P39216]
Paralogous protein(s)
[protein|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB], [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC], [protein|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB], [protein|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA]
[wiki|Localization]
cell membrane [Pubmed|21515776], Polar (Septum) [Pubmed|16479537]
accumulates at strongly curved membrane areas generated during cell division [Pubmed|26522943]
Expression and Regulation
Operons
genes
[gene|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]-[gene|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA]
description
[Pubmed|8188684]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8188684], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
additional information
in minimal medium, McpA is present with 15,900 +/- 3,000 molecules per cell [PubMed|21515776]
Biological materials
Mutant
BKE31250 ([gene|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT, downstream forward: _UP4_TAATAGGTCTAAAATACTAT
BKK31250 ([gene|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT, downstream forward: _UP4_TAATAGGTCTAAAATACTAT
References
Reviews
Original Publications
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Time of last update: 2024-12-21 15:00:48
Author of last update: Jstuelk