tlpA

tlpA
168

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, acts also as pH sensor, responds to decreasing pH as an attractant signal

Locus
BSU_31250
Molecular weight
72.09 kDa
Isoelectric point
4.91
Protein length
662 aaSequenceBlast
Gene length
1989 bpSequenceBlast
Function
control of chemotaxis, sensing of acidic environments
Product
methyl-accepting chemotaxis protein
Essential
no
Synonyms
tlpA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,208,280  3,210,268
The protein
Domains
Cache domain (aa 152-228) (according to UniProt)
HAMP domain (aa 303-355) (according to UniProt)
Methyl-accepting transducer domain (aa 374-610) (according to UniProt)
Structure
6S1K (PDB) (from E. coli, corresponds to aa 285 ... 552, 33% identity) PubMed
P39216 (AlphaFold)
Paralogous protein(s)
TlpB, McpC, McpB, McpA
Localization
cell membrane PubMed,  Polar (Septum) PubMed
accumulates at strongly curved membrane areas generated during cell division PubMed
Expression and Regulation
Operons
Genes
tlpA-mcpA
Description
PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
Additional information
in minimal medium, McpA is present with 15,900 +/- 3,000 molecules per cell PubMed
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tlpA→mcpA

2025-05-10 05:31:38

ghost

86

d93fadc070f0651f242c5fa096ab3f61c3c656b2

978EA1700C85B753C575F28E2A583FE04FD4E8F7

Biological materials
Mutant
BKE31250 (tlpA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT,  downstream forward: _UP4_TAATAGGTCTAAAATACTAT
BKK31250 (tlpA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT,  downstream forward: _UP4_TAATAGGTCTAAAATACTAT
References
Reviews
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Original Publications
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1DE26CDEE952142C1303C822F0E2A63AE09F721B

Page visits: 3292

Time of last update: 2025-05-15 19:01:01

Author of last update: Jstuelk