PGP3223
C-terminal Osmy fusion (from E.coli), without ATG or STOP; 6xHIS tag in between GOI and OsmY, TEV-cleavage site is in front of the His tag to remove the tag completely
Enzymes: NdeI for fwd and XmaI for rev
C-terminal Osmy fusion (from E.coli), without ATG or STOP; 6xHIS tag in between GOI and OsmY, TEV-cleavage site is in front of the His tag to remove the tag completely
Enzymes: NdeI for fwd and XmaI for rev