BioI

• Description: cytochrome P450 enzyme
  
  

Gene name	bioI
Synonyms
Essential	no
Product	cytochrome P450 enzyme
Function	biosynthesis of biotin
Gene expression levels in SubtiExpress: bioI
Interactions involving this protein in SubtInteract: BioI
Metabolic function and regulation of this protein in SubtiPathways: bioI
MW, pI	44 kDa, 6.2
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	ytbQ, bioB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed File:BioI expression.png

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

• Locus tag: BSU30190

Database entries

• BsubCyc: BSU30190

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids

• Protein family: cytochrome P450 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Fer-BioI PubMed
  • YkuP-BioI PubMed
  • AcpA-BioI PubMed

• Localization:

Database entries

• BsubCyc: BSU30190

• Structure: 3EJB, 3EJE (complex with AcpA) PubMed

• UniProt: P53554

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ PubMed

• Expression browser: bioI PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of biotin (BirA) PubMed

• Regulatory mechanism:
  • BirA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

<pubmed> 18838690 ,8763940,8892842,15449931, 15449930 12538057 20658980 11368323 11472016 14737344 12943422 </pubmed>