BioF

• Description: 8-amino-7-oxononanoate synthase
  
  

Gene name	bioF
Synonyms
Essential	no
Product	8-amino-7-oxononanoate synthase
Function	biosynthesis of biotin
Gene expression levels in SubtiExpress: bioF
Metabolic function and regulation of this protein in SubtiPathways: bioF
MW, pI	42 kDa, 6.285
Gene length, protein length	1167 bp, 389 aa
Immediate neighbours	bioD, bioA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

• Locus tag: BSU30220

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO₂ (according to Swiss-Prot)

• Protein family: class-II pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P53556

• KEGG entry: [3]

• E.C. number: 2.3.1.47

Expression and regulation

• Operon: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ PubMed

• Expression browser: bioF PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of biotin (BirA) PubMed

• Regulatory mechanism:
  • BirA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

<pubmed>8763940,8892842,21821766,22960854 </pubmed>