Revision as of 12:48, 13 May 2013

Description: malonyl CoA-acyl carrier protein transacylase

Gene name	fabD
Synonyms	ylpE
Essential	yes PubMed
Product	malonyl CoA-acyl carrier protein transacylase
Function	fatty acid biosynthesis
*Gene expression levels in SubtiExpress*: fabD
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	33 kDa, 4.515
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	plsX, fabG
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes

This gene is a member of the following regulons

ComA regulon, FapR regulon

The gene

Basic information

Locus tag: BSU15900

Phenotypes of a mutant

essential PubMed

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity: Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein] (according to Swiss-Prot)

Protein family: fabD family (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization:

Database entries

Structure: 1MLA (the enzyme from E. coli) PubMed

UniProt: P71019

KEGG entry: [3]

E.C. number: 2.3.1.39

Additional information

Expression and regulation

Operon: fapR-plsX-fabD-fabG-acpA PubMed

Expression browser: fabD PubMed

Sigma factor: SigA PubMed

Regulation:
- induced if the cells experience an accumulation of malonyl-CoA (FapR) PubMed
- expressed at high cell density (ComA) PubMed

Regulatory mechanism:
- ComA: transcription activation (ComA) PubMed
- FapR: transcription repression PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU15900 fabD]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15900 fabD]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[plsX]]'', ''[[fabG]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13463&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15900 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15900 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15900 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:fabD_context.gif]]