Difference between revisions of "ThiM"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU38300 thiM]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38300 thiM]
 
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|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiE]]'', ''[[ywbI]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiE]]'', ''[[ywbI]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15856&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38300 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38300 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38300 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:thiM_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:thiM_context.gif]]

Revision as of 14:10, 13 May 2013

  • Description: hydroxyethylthiazole kinase

Gene name thiM
Synonyms thiK, ipa-25d, ywbJ
Essential no
Product hydroxyethylthiazole kinase
Function biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiM
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 28 kDa, 5.393
Gene length, protein length 816 bp, 272 aa
Immediate neighbours thiE, ywbI
Sequences Protein DNA Advanced_DNA
Genetic context
ThiM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiM expression.png




























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU38300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole (according to Swiss-Prot)
  • Protein family: Thz kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

N Campobasso, I I Mathews, T P Begley, S E Ealick
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.
Biochemistry: 2000, 39(27);7868-77
[PubMed:10891066] [WorldCat.org] [DOI] (P p)

Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923] [WorldCat.org] [DOI] (P p)