AsnO

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  • Description: asparagine synthase (glutamine-hydrolysing)

Gene name asnO
Synonyms yisO, yucB
Essential no
Product asparagine synthase (glutamine-hydrolysing)
Function biosynthesis of asparagine
Gene expression levels in SubtiExpress: asnO
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 70 kDa, 5.696
Gene length, protein length 1842 bp, 614 aa
Immediate neighbours yisN, yizA
Sequences Protein DNA Advanced_DNA
Genetic context
AsnO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AsnO expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU10790

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)
  • Protein family: glutamine amidotransferase type-2 domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721] [WorldCat.org] [DOI] (P p)