IolE

• Description: 2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)
  
  

Gene name	iolE
Synonyms	yxdE
Essential	no
Product	2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)
Function	myo-inositol catabolism
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	33 kDa, 5.209
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	iolF, iolD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39720

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H₂O (according to Swiss-Prot)

• Protein family: iolE/mocC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P42416

• KEGG entry: [3]

• E.C. number: 4.2.1.44

Expression and regulation

• Operon: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the absence of inositol (IolR) PubMed
  • repressed by glucose (CcpA) PubMed
  • induced by inositol (IolR) PubMed

• Regulatory mechanism:
  • IolR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • IolR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• Ken-ichi Yoshida, Kobe University, Japan

References

1. Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y. (2008) myo-Inositol catabolism in Bacillus subtilis. J Biol Chem. Apr 18;283(16): 10415-24. PubMed
2. Yoshida K., Yamaguchi M., Ikeda H., Omae K., Tsurusaki K., Fujita Y. (2004) The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol dehydratase. Microbiology Mar;150(Pt 3): 571-80. PubMed