iolB

168

formation of 2-deoxy-5-keto-gluconic acid (4th reaction)

Locus: BSU_39750

Molecular weight: 30.62 kDa

Isoelectric point: 4.77

Protein length: 271 aa

Gene length: 816 bp

Function: myo-inositol catabolism

Product: formation of 2-deoxy-5-keto-gluconic acid (4th reaction)

Essential: no

E.C.: null

Synonyms: iolB, yxdB

Genomic Context

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List of homologs in different organisms, belongs to COG3718 (Galperin et al., 2021)

Gene

Coordinates: 4,082,030  4,082,845

The protein

Catalyzed reaction/ biological activity

• 5-deoxy-D-glucuronate --> 5-dehydro-2-deoxy-D-gluconate (according to UniProt)

Protein family

• isomerase IolB family (single member, according to UniProt)

Structure

• 2QJV (PDB) (from Salmonella typhimurium, 49% identity, 65% similarity)
• P42413 (AlphaFold)

Expression and Regulation

Operons

• Genes: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ

  Description: PubMed

  Regulation

  • induced by inositol (IolR) PubMed

  Regulatory mechanism

  • IolR: repression, PubMed, in iolR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B771 (iolB::erm), available at the NBRP B. subtilis, Japan
• BKE39750 (iolB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGAGAAGCCCTCCTCTT,  downstream forward: _UP4_TAACAAGTGAGGAGTGGCTG
• BKK39750 (iolB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGAGAAGCCCTCCTCTT,  downstream forward: _UP4_TAACAAGTGAGGAGTGGCTG

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

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