IolG

• Description: inositol 2-dehydrogenase
  
  

Gene name	iolG
Synonyms	idh, iol
Essential	no
Product	inositol 2-dehydrogenase
Function	myo-inositol catabolism
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	38 kDa, 4.865
Gene length, protein length	1032 bp, 344 aa
Immediate neighbours	iolH, iolF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39700

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Myo-inositol + NAD⁺ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH (according to Swiss-Prot)

• Protein family: gfo/idh/mocA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P26935

• KEGG entry: [3]

• E.C. number: 1.1.1.18

Expression and regulation

• Operon: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the absence of inositol (IolR) PubMed
  • repressed by glucose (CcpA) PubMed
  • induced by inositol (IolR) PubMed

• Regulatory mechanism:
  • IolR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • IolR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• Ken-ichi Yoshida, Kobe University, Japan

References

1. Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y. (2008) myo-Inositol catabolism in Bacillus subtilis. J Biol Chem. Apr 18;283(16): 10415-24. PubMed
2. Ramaley R, Fujita Y, Freese E. (1979) Purification and properties of Bacillus subtilis inositol dehydrogenase. J Biol Chem. Aug 25;254(16): 7684-90. PubMed