GmuE

• Description: mannose kinase
  
  

Gene name	gmuE
Synonyms	ydhR
Essential	no
Product	mannose kinase
Function	glucomannan utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	32 kDa, 5.013
Gene length, protein length	897 bp, 299 aa
Immediate neighbours	gmuR, gmuF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU05860

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-fructose = ADP + D-fructose 6-phosphate (according to Swiss-Prot)

• Protein family: ROK (nagC/xylR) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1XC3

• Swiss prot entry: O05510

• KEGG entry: [3]

• E.C. number: 2.7.1.4

Expression and regulation

• Operon: gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by conjac glucomannan (requires GmuG) (GmuR) PubMed
  • induced by cellobiose (GmuR) PubMed
  • subject to carbon catabolite repression (CcpA) PubMed

• Regulatory mechanism:
  • GmuR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
2. Sadaie Y, Nakadate H, Fukui R, Yee LM, Asai K. (2008) Glucomannan utilization operon of Bacillus subtilis. FEMS Microbiol Lett. Feb;279(1): 103-9. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed