gmuG

168

beta-1,4-mannanase

Locus: BSU_05880

Molecular weight: 40.68 kDa

Isoelectric point: 5.63

Protein length: 362 aa

Gene length: 1089 bp

Function: glucomannan utilization

Product: beta-1,4-mannanase

Essential: no

E.C.: 3.2.1.78

Synonyms: gmuG, ydhT

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4124 (Galperin et al., 2021)

Gene

Coordinates: 632,774  633,862

The protein

Catalyzed reaction/ biological activity

• Random hydrolysis of (14)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans (according to UniProt)

Protein family

• glycosyl hydrolase 26 family (single member, according to UniProt)

Domains

• GH26 domain (aa 38-349) (according to UniProt)

Structure

• 2QHA (PDB), 3CBW mutant variant
• O05512 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG

  Description: PubMed

  Regulation

  • induced by cellobiose (GmuR) PubMed

  Regulatory mechanism

  • GmuR: repression, PubMed, in gmuR regulon
  • CcpA: repression, PubMed, in ccpA regulon
  • AbrB: activation, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C195 (ydhT::erm), available at the NBRP B. subtilis, Japan
• BKE05880 (gmuG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATGCAACTCCCCCATTC,  downstream forward: _UP4_TGAATCCGTAATGTCACAAT
• BKK05880 (gmuG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATGCAACTCCCCCATTC,  downstream forward: _UP4_TGAATCCGTAATGTCACAAT

References

Loading