PelB

• Description: pectate lyase
  
  

Gene name	pelB
Synonyms	ppr
Essential	no
Product	pectate lyase
Function	degradation of polygalacturonic acid
MW, pI	37 kDa, 9.436
Gene length, protein length	1035 bp, 345 aa
Immediate neighbours	yoaK, yoaM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU18650

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to Swiss-Prot)

• Protein family: UPF0700 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure:

• Swiss prot entry: O34819

• KEGG entry: [2]

• E.C. number: 4.2.2.10

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed