pelB

168

pectate lyase

Locus: BSU_18650

Molecular weight: 37.95 kDa

Isoelectric point: 9.44

Protein length: 345 aa

Gene length: 1038 bp

Function: degradation of polygalacturonic acid

Product: pectate lyase

Essential: no

E.C.: 4.2.2.10

Synonyms: pelB, ppr

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3866 (Galperin et al., 2021)

Gene

Coordinates: 2,034,745  2,035,782

The protein

Catalyzed reaction/ biological activity

• Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to UniProt)

Protein family

• lyase 1 family (according to UniProt)

Structure

• 3VMV (PDB) (from Bacillus sp. N165, 29% identity) PubMed
• O34819 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Biological materials

Mutant

• BKE18650 (pelB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACAATATGCTTCCTTT,  downstream forward: _UP4_TGATATAAAAGAGGCCCTGC
• BKK18650 (pelB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACAATATGCTTCCTTT,  downstream forward: _UP4_TGATATAAAAGAGGCCCTGC

References

Loading