Obg
- Description: GTP-binding protein involved in initiation of sporulation
Gene name | obg |
Synonyms | |
Essential | Yes |
Product | GTP-binding protein |
Function | may be required to stimulate activity of the phosphorelay that activates Spo0A |
MW, pI | 47 kDa, 4.859 |
Gene length, protein length | 1284 bp, 428 aa |
Immediate neighbours | spo0B, pheB |
Gene sequence (+200bp) | Protein sequence |
Genetic context |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family: Era/Obg family
Extended information on the protein
- Kinetic information:
- Domains:
Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
- Modification:
- Cofactor(s):
GDP, GTP and probably ppGpp
- Effectors of protein activity:
- Interactions:
Interaction with Rsb proteins.
- Localization:
Database entries
- Structure:1LNZ
- Swiss prot entry:[3]
- KEGG entry:[4]
- E.C. number:
Additional information
The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.
Expression and regulation
- Sigma factor: SigA
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Buglino J., Shen V., Hakiman P. & Lima C.D. (2002) Structural and biochemical analysis of the Obg GTP binding protein Structure 10: 1581-1592. PubMed
- Scott, J.M., Ju, J., Mitchell, T., and Haldenwang, W.G. (2000) The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes J. Bacteriol. 182: 2771–2777. PubMed