IlvD

• Description: dihydroxy-acid dehydratase (2,3-dihydroxy-3-methylbutanoate, 2,3-dihydroxy-3-methylpentanoate)
  
  

Gene name	ilvD
Synonyms
Essential	no
Product	dihydroxy-acid dehydratase (2,3-dihydroxy-3-methylbutanoate, 2,3-dihydroxy-3-methylpentanoate)
Function	biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ilvD
Metabolic function and regulation of this protein in SubtiPathways: ilvD
MW, pI	59 kDa, 5.315
Gene length, protein length	1674 bp, 558 aa
Immediate neighbours	brxA, ypgR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, most abundant proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU21870

Database entries

• BsubCyc: BSU21870

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H₂O (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU21870

• Structure: http://www.rcsb.org/pdb/explore/explore.do?structureId=2GP4 2GP4 (apo form of 6-phosphogluconate dehydratase from Shewanella oneidensis, 33% identity)

• UniProt: P51785

• KEGG entry: [3]

• E.C. number: 4.2.1.9

Expression and regulation

• Operon: ilvD PubMed

• Expression browser: ilvD PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed1 PubMed2

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 3104 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1545 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4271 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1398 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2083 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP522 (in pAC5), pGP235 (in pAC5), both available in Jörg Stülke's lab; a series of promoter deletions in pAC6 is available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system:

• Antibody: