ilvD

168

dihydroxy-acid dehydratase (2,3-dihydroxy-3-methylbutanoate, 2,3-dihydroxy-3-methylpentanoate)

Locus: BSU_21870

Molecular weight: 59.38 kDa

Isoelectric point: 5.32

Protein length: 558 aa

Gene length: 1677 bp

Function: biosynthesis of branched-chain amino acids

Product: dihydroxy-acid dehydratase (2,3-dihydroxy-3-methylbutyrate, 2,3-dihydroxy-3-methylpentanoate)

Essential: no

E.C.: 4.2.1.9

Synonyms: ilvD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0129 (Galperin et al., 2021)

Gene

Coordinates: 2,300,762 2,302,438

The protein

Catalyzed reaction/ biological activity

• 2,3-dihydroxy-3-methylbutyrate --> 3-methyl-2-oxobutyrate + H₂O(according to UniProt)

Protein family

• IlvD/Edd family (single member, according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 2GP4 (PDB) (apo form of 6-phosphogluconate dehydratase from Shewanella oneidensis, 33% identity)
• P51785 (AlphaFold)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ilvD

  Description: PubMed

  Regulation

  • repressed by casamino acids PubMed

  Regulatory mechanism

  • CodY: repression, PubMed PubMed, in codY regulon

Biological materials

Mutant

• BKE21870 (ilvD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTGATCCTCCTAAATT, downstream forward: _UP4_TAGATAGCTGGGGACACTTT
• BKK21870 (ilvD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTGATCCTCCTAAATT, downstream forward: _UP4_TAGATAGCTGGGGACACTTT

LacZ fusion

• pGP522 (in pAC5), pGP235 (in pAC5), both available in Jrg Stlke's lab
• a series of promoter deletions in pAC6 is available in Jrg Stlke's lab

References

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