ComGC

• Description: DNA uptake, major component of the pseudopilus
  
  

Gene name	comGC
Synonyms
Essential	no
Product	major pseudopilin
Function	genetic competence
Gene expression levels in SubtiExpress: comGC
Interactions involving this protein in SubtInteract: ComGC
Metabolic function and regulation of this protein in SubtiPathways: ComGC
MW, pI	10 kDa, 6.325
Gene length, protein length	294 bp, 98 aa
Immediate neighbours	comGD, comGB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU24710

Phenotypes of a mutant

• knockout has complete loss of transformation competence

Database entries

• BsubCyc: BSU24710

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: comGC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ComGB-ComGC PubMed
  • ComC-ComGC PubMed
  • forms large complexes (the pseudopilus) PubMed

• Localization:
  • cell surface PubMed
  • localizes to the cell membrane and the cytoplasm PubMed
  • localizes to one single cell pole in the majority of the cells PubMed

= Database entries

• Structure:

• UniProt: P25955

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comGA-comGB-comGC-comGD-comGE-comGF-comGG-yqzE PubMed

• Expression browser: comGC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)