Amj

• Description: lipid II flippase, alternative to MurJ
  
  

Gene name	ydaH
Synonyms
Essential	no
Product	lipid II flippase
Function	export of lipid II
Gene expression levels in SubtiExpress: amj
MW, pI	29 kDa, 10.596
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	bsrC, ydzA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU04230

Phenotypes of a mutant

• an amj murJ double mutant is not viable PubMed

Database entries

• BsubCyc: BSU04230

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU04230

• Structure:

• UniProt: P96581

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ydaH PubMed

• Expression browser: amj PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alexander J Meeske, Lok-To Sham, Harvey Kimsey, Byoung-Mo Koo, Carol A Gross, Thomas G Bernhardt, David Z Rudner
MurJ and a novel lipid II flippase are required for cell wall biogenesis in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2015, 112(20);6437-42
[PubMed:25918422] [WorldCat.org] [DOI] (I p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)