murJ

168

lipid II flippase

Locus: BSU_30050

Molecular weight: 59.30 kDa

Isoelectric point: 10.09

Protein length: 544 aa

Gene length: 1635 bp

Function: export of lipid II

Product: lipid II flippase

Essential: no

Synonyms: murJ, ytgP

Genomic Context

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List of homologs in different organisms, belongs to COG5841 (Galperin et al., 2021)

Gene

Coordinates: 3,073,531  3,075,165

Phenotypes of a mutant

• an ''amj murJ'' double mutant is not viable PubMed
• a ''sigM murJ'' double mutant is not viable (due to the lack of both lipid II flippases) PubMed
• mutants lacking MurJ produce long cells and chains of cells  PubMed

The protein

Protein family

• Polysaccharide synthase family (according to UniProt)
• MOP exporter family

Structure

• 5T77 (PDB) (from Thermosipho africanus, 20% identity) PubMed
• O34674 (AlphaFold)

Paralogous protein(s)

• YkvU, SpoVB, YabM

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: murJ-ytzG

  Description: PubMed

Biological materials

Mutant

• MGNA-A012 (ytgP::erm), available at the NBRP B. subtilis, Japan
• BKE30050 (murJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTCTTCATCACCATT,  downstream forward: _UP4_TGATGAACTAGATATGATGA
• BKK30050 (murJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTCTTCATCACCATT,  downstream forward: _UP4_TGATGAACTAGATATGATGA

References

Reviews

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Original Publications

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