GlyQ

• Description: glycyl-tRNA synthetase (alpha subunit)
  
  

Gene name	glyQ
Synonyms	yqfJ
Essential	yes PubMed
Product	glycyl-tRNA synthetase (alpha subunit)
Function	translation
Gene expression levels in SubtiExpress: glyQ
Interactions involving this protein in SubtInteract: GlyQ
Metabolic function and regulation of this protein in SubtiPathways: glyQ
MW, pI	33 kDa, 4.8
Gene length, protein length	885 bp, 295 aa
Immediate neighbours	glyS, recO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, most abundant proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU25270

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25270

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions: GlyQ-GlyS

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU25270

• Structure: 1J5W (from Thermotoga maritima, 55% identity, 72% similarity)

• UniProt: P54380

• KEGG entry: [3]

• E.C. number: 6.1.1.14

Expression and regulation

• Operon: glyQ-glyS (according to DBTBS)

• Expression browser: glyQ PubMed

• Sigma factor:

• Regulation:
  • induced by glycine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 821 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1704 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1622 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1214 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1611 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jinwei Zhang, Adrian R Ferré-D'Amaré
Direct evaluation of tRNA aminoacylation status by the T-box riboswitch using tRNA-mRNA stacking and steric readout.
Mol Cell: 2014, 55(1);148-55
[PubMed:24954903] [WorldCat.org] [DOI] (I p)

Jason C Grigg, Yujie Chen, Frank J Grundy, Tina M Henkin, Lois Pollack, Ailong Ke
T box RNA decodes both the information content and geometry of tRNA to affect gene expression.
Proc Natl Acad Sci U S A: 2013, 110(18);7240-5
[PubMed:23589841] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Audrey R Nelson, Tina M Henkin, Paul F Agris
tRNA regulation of gene expression: interactions of an mRNA 5'-UTR with a regulatory tRNA.
RNA: 2006, 12(7);1254-61
[PubMed:16741230] [WorldCat.org] [DOI] (P p)

Mary R Yousef, Frank J Grundy, Tina M Henkin
Structural transitions induced by the interaction between tRNA(Gly) and the Bacillus subtilis glyQS T box leader RNA.
J Mol Biol: 2005, 349(2);273-87
[PubMed:15890195] [WorldCat.org] [DOI] (P p)

Frank J Grundy, Mary R Yousef, Tina M Henkin
Monitoring uncharged tRNA during transcription of the Bacillus subtilis glyQS gene.
J Mol Biol: 2005, 346(1);73-81
[PubMed:15663928] [WorldCat.org] [DOI] (P p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

Frank J Grundy, Tina M Henkin
Kinetic analysis of tRNA-directed transcription antitermination of the Bacillus subtilis glyQS gene in vitro.
J Bacteriol: 2004, 186(16);5392-9
[PubMed:15292140] [WorldCat.org] [DOI] (P p)

Mary R Yousef, Frank J Grundy, Tina M Henkin
tRNA requirements for glyQS antitermination: a new twist on tRNA.
RNA: 2003, 9(9);1148-56
[PubMed:12923262] [WorldCat.org] [DOI] (P p)