Noc

• Description: DNA-binding protein, spatial regulator of cell division to protect the nucleoid, and timing device with an important role in the coordination of chromosome segregation and cell division, Noc and the Min system ensure the efficient utilization of the division site at midcell in by ensuring Z ring placement
  
  

Gene name	noc
Synonyms	yyaA
Essential	no
Product	effector of nucleoid occlusion
Function	control of cell division
Gene expression levels in SubtiExpress: noc
MW, pI	32 kDa, 5.812
Gene length, protein length	849 bp, 283 aa
Immediate neighbours	yyaB, rsmG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, membrane proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU40990

Phenotypes of a mutant

• a yvcL noc double mutant grows poorly, and the cells are filamentous PubMed

Database entries

• BsubCyc: BSU40990

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds specific sites on the chromosome PubMed

• Protein family: ParB family (according to Swiss-Prot)

• Paralogous protein(s): Spo0J

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • associated to the cell membrane PubMed

Database entries

• BsubCyc: BSU40990

• Structure:

• UniProt: P37524

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: thdF-gidA-rsmG-noc PubMed

• Expression browser: noc PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 382 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 7544 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion: available in the Jeff Errington lab

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle, UK Homepage

References

Reviews

Marc Bramkamp, Suey van Baarle
Division site selection in rod-shaped bacteria.
Curr Opin Microbiol: 2009, 12(6);683-8
[PubMed:19884039] [WorldCat.org] [DOI] (I p)

David W Adams, Jeff Errington
Bacterial cell division: assembly, maintenance and disassembly of the Z ring.
Nat Rev Microbiol: 2009, 7(9);642-53
[PubMed:19680248] [WorldCat.org] [DOI] (I p)

Original Publications

David William Adams, Ling Juan Wu, Jeff Errington
Nucleoid occlusion protein Noc recruits DNA to the bacterial cell membrane.
EMBO J: 2015, 34(4);491-501
[PubMed:25568309] [WorldCat.org] [DOI] (I p)

Katarina Surdova, Pamela Gamba, Dennis Claessen, Tjalling Siersma, Martijs J Jonker, Jeff Errington, Leendert W Hamoen
The conserved DNA-binding protein WhiA is involved in cell division in Bacillus subtilis.
J Bacteriol: 2013, 195(24);5450-60
[PubMed:24097947] [WorldCat.org] [DOI] (I p)

Erik Nico Trip, Jan-Willem Veening, Eric J Stewart, Jeff Errington, Dirk-Jan Scheffers
Balanced transcription of cell division genes in Bacillus subtilis as revealed by single cell analysis.
Environ Microbiol: 2013, 15(12);3196-209
[PubMed:23701187] [WorldCat.org] [DOI] (I p)

Christopher D A Rodrigues, Elizabeth J Harry
The Min system and nucleoid occlusion are not required for identifying the division site in Bacillus subtilis but ensure its efficient utilization.
PLoS Genet: 2012, 8(3);e1002561
[PubMed:22457634] [WorldCat.org] [DOI] (I p)

Ling Juan Wu, Shu Ishikawa, Yoshikazu Kawai, Taku Oshima, Naotake Ogasawara, Jeff Errington
Noc protein binds to specific DNA sequences to coordinate cell division with chromosome segregation.
EMBO J: 2009, 28(13);1940-52
[PubMed:19494834] [WorldCat.org] [DOI] (I p)

Yoshikazu Kawai, Naotake Ogasawara
Bacillus subtilis EzrA and FtsL synergistically regulate FtsZ ring dynamics during cell division.
Microbiology (Reading): 2006, 152(Pt 4);1129-1141
[PubMed:16549676] [WorldCat.org] [DOI] (P p)

Ling Juan Wu, Jeff Errington
Coordination of cell division and chromosome segregation by a nucleoid occlusion protein in Bacillus subtilis.
Cell: 2004, 117(7);915-25
[PubMed:15210112] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Jörg Sievers, Brian Raether, Marta Perego, Jeff Errington
Characterization of the parB-like yyaA gene of Bacillus subtilis.
J Bacteriol: 2002, 184(4);1102-11
[PubMed:11807071] [WorldCat.org] [DOI] (P p)