PhoR

• Description: two-component sensor kinase, regulation of phosphate metabolism
  
  

Gene name	phoR
Synonyms
Essential	no
Product	two-component sensor kinase
Function	regulation of phosphate metabolism
Gene expression levels in SubtiExpress: phoR
Interactions involving this protein in SubtInteract: PhoR
MW, pI	64 kDa, 5.957
Gene length, protein length	1737 bp, 579 aa
Immediate neighbours	polA, phoP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism, protein modification, transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, SigB regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU29100

Database entries

• BsubCyc: BSU29100

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of PhoP

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • two transmembrane segments
  • PAS domain, for binding of an intermediate of wall teichoic acid biosynthesis PubMed
  • C-terminal histidine phosphotransferase domain

• Modification:
  • autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular PAS domain of PhoR PubMed

• Cofactor(s):

• Effectors of protein activity:
  • activity is inhibited by binding of an intermediate of wall teichoic acid biosynthesis to the intracellular PAS domain of PhoR PubMed

• Interactions:
  • PhoP-PhoR

• Localization:

Database entries

• BsubCyc: BSU29100

• Structure: 3CWF (extracellular domain) PubMed

• UniProt: P23545

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: phoP-phoR PubMed

• Expression browser: phoR PubMed

• Sigma factor: SigA PubMed, SigB PubMed, SigE PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed
  • expressed in post-exponential phase (ScoC) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • PhoP: transcription activation PubMed
  • ScoC: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 453 PubMed

Biological materials

• Mutant:
  • 1A966 ( phoR::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

References

Reviews

Georg Fritz, Thorsten Mascher
A balancing act times two: sensing and regulating cell envelope homeostasis in Bacillus subtilis.
Mol Microbiol: 2014, 94(6);1201-7
[PubMed:25355628] [WorldCat.org] [DOI] (I p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)