SpoIIQ

• Description: component of the SpoIIIAH-SpoIIQ type III secretion system residing in the forespore membrane, required for anchoring of proteins on both sides of the sporulation septum
  
  

Gene name	spoIIQ
Synonyms	ywnI
Essential	no
Product	part of the transmembrane channel linking the mother cell and the forespore
Function	forespore encasement by the spore coat
Gene expression levels in SubtiExpress: spoIIQ
Interactions involving this protein in SubtInteract: SpoIIQ
MW, pI	30 kDa, 4.475
Gene length, protein length	849 bp, 283 aa
Immediate neighbours	ywnJ, ywnH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU36550

Database entries

• BsubCyc: BSU36550

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for forespore encasement by the spore coat PubMed
  • required for the recruitment of SpoIIIAH to the sporulation septum on the mother cell side PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIIIAH(12)-SpoIIQ(12) (interaction between proteins membrane proteins expressed in the mother cell and the forespore, respectively; the complex forms a 60 Å channel made up of twelve subunits of each protein) PubMed
  • SpoIIQ-SpoIIE

• Localization:
  • membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with SpoIIIAH) PubMed
  • proper localization requires SpoIIIAH and degradation of septal peptidoglycan by the cell wall hydrolases SpoIID and SpoIIP PubMed

Database entries

• BsubCyc: BSU36550

• Structure:
  • 3UZO; 3TUF (the SpoIIQ-SpoIIIAH pore forming complex) PubMed

• UniProt: P71044

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIQ PubMed

• Expression browser: spoIIQ PubMed

• Sigma factor: SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Adam D Crawshaw, Mónica Serrano, Will A Stanley, Adriano O Henriques, Paula S Salgado
A mother cell-to-forespore channel: current understanding and future challenges.
FEMS Microbiol Lett: 2014, 358(2);129-36
[PubMed:25105965] [WorldCat.org] [DOI] (I p)

Lotte Søgaard-Andersen
Stably bridging a great divide: localization of the SpoIIQ landmark protein in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1019-24
[PubMed:23944268] [WorldCat.org] [DOI] (I p)