RadC

• Description: probable DNA repair protein
  
  

Gene name	radC
Synonyms	ysxA
Essential	no
Product	probable DNA repair protein
Function	DNA repair
Gene expression levels in SubtiExpress: radC
MW, pI	25 kDa, 7.829
Gene length, protein length	693 bp, 231 aa
Immediate neighbours	mreB, maf
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence, cell envelope stress proteins (controlled by SigM, V, W, X, Y), phosphoproteins

This gene is a member of the following regulons

ComK regulon, SigM regulon

The gene

Basic information

• Locus tag: BSU28040

Database entries

• BsubCyc: BSU28040

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: radC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-59 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• BsubCyc: BSU28040

• Structure:

• UniProt: Q02170

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: radC (according to DBTBS), radC-mreB-mreC-mreD-minC-minD PubMed

• Expression browser: radC PubMed

• Sigma factor: SigM PubMed

• Regulation:
  • expressed in response to cell wall antibiotics, ethanol, heat, acid, and superoxide stress (SigM) PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Your additional remarks

The radC locus is the chromosomal integration site for the φ105 prophage Pubmed

References

Additional publications: PubMed

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Penny D Thackray, Anne Moir
SigM, an extracytoplasmic function sigma factor of Bacillus subtilis, is activated in response to cell wall antibiotics, ethanol, heat, acid, and superoxide stress.
J Bacteriol: 2003, 185(12);3491-8
[PubMed:12775685] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Randy M Berka, Jeanette Hahn, Mark Albano, Irena Draskovic, Marjan Persuh, Xianju Cui, Alan Sloma, William Widner, David Dubnau
Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK.
Mol Microbiol: 2002, 43(5);1331-45
[PubMed:11918817] [WorldCat.org] [DOI] (P p)