DegQ

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  • Description: stimulates production of degradative enzymes and of extracellular poly-gamma-glutamate PubMed, stimulates phosphorylation of DegU by DegS PubMed, gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed

Gene name degQ
Synonyms sacQ
Essential no
Product pleiotropic regulator
Function regulation of exoenzyme synthesis
Gene expression levels in SubtiExpress: degQ
Interactions involving this protein in SubtInteract: DegQ
MW, pI 5 kDa, 6.536
Gene length, protein length 138 bp, 46 aa
Immediate neighbours comQ, yuzC
Sequences Protein DNA DNA_with_flanks
Genetic context
DegQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DegQ expression.png
























Categories containing this gene/protein

biofilm formation, transcription factors and their control, sporulation proteins

This gene is a member of the following regulons

ComA regulon, DegU regulon

The gene

Basic information

  • Locus tag: BSU31720

Phenotypes of a mutant

loss of poly-gamma-glutamate production PubMed, no synthesis of nonribosomal peptides iturin A and plipastatin PubMed1 PubMed2, reduced synthesis of degradative enzymes PubMed, increased genetic competence PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: degQ family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information: the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed

Biological materials

  • Mutant: QB4851 (cat), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Anna L McLoon, Sarah B Guttenplan, Daniel B Kearns, Roberto Kolter, Richard Losick
Tracing the domestication of a biofilm-forming bacterium.
J Bacteriol: 2011, 193(8);2027-34
[PubMed:21278284] [WorldCat.org] [DOI] (I p)

Nobuchika Takesue, Teruo Sone, Michiko Tanaka, Fusao Tomita, Kozo Asano
Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system.
J Biosci Bioeng: 2009, 107(6);623-9
[PubMed:19447339] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)

Nicola R Stanley, Beth A Lazazzera
Defining the genetic differences between wild and domestic strains of Bacillus subtilis that affect poly-gamma-dl-glutamic acid production and biofilm formation.
Mol Microbiol: 2005, 57(4);1143-58
[PubMed:16091050] [WorldCat.org] [DOI] (P p)

T Msadek, F Kunst, A Klier, G Rapoport
DegS-DegU and ComP-ComA modulator-effector pairs control expression of the Bacillus subtilis pleiotropic regulatory gene degQ.
J Bacteriol: 1991, 173(7);2366-77
[PubMed:1901055] [WorldCat.org] [DOI] (P p)

H Shimotsu, D J Henner
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
J Bacteriol: 1986, 168(1);380-8
[PubMed:2428811] [WorldCat.org] [DOI] (P p)

M Yang, E Ferrari, E Chen, D J Henner
Identification of the pleiotropic sacQ gene of Bacillus subtilis.
J Bacteriol: 1986, 166(1);113-9
[PubMed:3007431] [WorldCat.org] [DOI] (P p)